Coding

Part:BBa_K2088007

Designed by: Yuxin He   Group: iGEM16_NAU-CHINA   (2016-10-08)


pbaB: a 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain.

PbaB is a [2Fe-2S]-type ferredoxin, found in Sphingobium wenxiniae JZ-1.It can express in E.coli(DH5ɑ or BL21) in plasmid pPBA. This part encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions.

Mostly, pbaB implements its function with pbaA1A2(BBa_K2088003). PbaA1A2B are the oxygenase and ferredoxin components of the angular dioxygenase (3-phenoxybenzoate 1’,2’-dioxygenase) responsible for the angular dioxygenation at the 1’ and 2’ positions on the benzene moiety of 3-phenoxybenzoate and the ferredoxin PbaB is indispensable for the angular dioxygenase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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